Pdpn^tm1Mcws
Targeted Allele Detail

Summary

• Symbol: Pdpn^tm1Mcws
• Name: podoplanin; targeted mutation 1, Mary C Williams
• MGI ID: MGI:2657021
• Synonyms: Gp38^tm1Mir, T1alpha-
• Gene: Pdpn Location: Chr4:142994001-143026134 bp, - strand Genetic Position: Chr4, 77.29 cM
• Alliance: Pdpn^tm1Mcws page

Lung abnormalities become apparent after E15.5 in Pdpn^tm1Mcws/Pdpn^tm1Mcws mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82687
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 5' UTR, exon 1, and a portion of intron 1 were replaced with a neomycin selection cassette inserted by homologous recombination. Neither transcript nor protein was detected in homozygous mutant mice by Northern and Western blot analyses of neonatal lung tissue. RT-PCR analysis confirmed the absence of transcript in homozygous mutant brain and kidney tissue. (J:82687)

Expression

In Mice Carrying this Mutation:	30 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdpn Mutation: 29 strains or lines available

References

• Original: J:82687 Ramirez MI, et al., T1alpha, a lung type I cell differentiation gene, is required for normal lung cell proliferation and alveolus formation at birth. Dev Biol. 2003 Apr 1;256(1):61-72
• All: 6 reference(s)