Ace^tm3Keb
Targeted Allele Detail

Summary

• Symbol: Ace^tm3Keb
• Name: angiotensin I converting enzyme; targeted mutation 3, Kenneth E Bernstein
• MGI ID: MGI:2657140
• Synonyms: ACE.3^-
• Gene: Ace Location: Chr11:105858774-105880790 bp, + strand Genetic Position: Chr11, 68.84 cM
• Alliance: Ace^tm3Keb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83178
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutation: Insertion
• Mutation details: A neo cassette immediately followed by a 2.3 kb albumin promoter/enhancer was inserted downstream of the endogenous somatic promoter and upstream of exon 1 by homologous recombination. The targeting construct was designed such that transcription of the somatic isoform would be controlled by the inserted albumin promoter, which drives expression in the liver. Protein was undetected in all but two of the somatic tissues tested. There was 87-fold increase in the hepatic protein level whereas the renal protein level was approximately 14% that of wild-type mice. The expression of the testes isoform was unaffected. (J:83178)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace Mutation: 57 strains or lines available

References

• Original: J:83178 Cole J, et al., Mice lacking endothelial angiotensin-converting enzyme have a normal blood pressure. Circ Res. 2002 Jan 11;90(1):87-92
• All: 8 reference(s)