Thrb^tm2Df
Targeted Allele Detail

Summary

• Symbol: Thrb^tm2Df
• Name: thyroid hormone receptor beta; targeted mutation 2, Douglas Forrest
• MGI ID: MGI:2657173
• Synonyms: Thrb2^-
• Gene: Thrb Location: Chr14:4431611-4809435 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A3
• Alliance: Thrb^tm2Df page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66737
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of 165 bp of coding sequence with a lacZ-neo cassette via homologous recombination resulting in the fusion of the first 54 amino acids to the lacZ gene. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of RNA from the pituitary and eyes. (J:66737)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thrb Mutation: 41 strains or lines available

References

• Original: J:66737 Ng L, et al., A thyroid hormone receptor that is required for the development of green cone photoreceptors. Nat Genet. 2001 Jan;27(1):94-8
• All: 10 reference(s)