Sptbn4^{Gt(ROSABetageo)62Sor}
Gene trapped Allele Detail

Summary

• Symbol: Sptbn4^{Gt(ROSABetageo)62Sor}
• Name: spectrin beta, non-erythrocytic 4; gene trap 62, Philippe Soriano
• MGI ID: MGI:2657204
• Synonyms: ROSA 62, sigma1sigma6^-, Spnb4^{Gt(ROSABgeo*)62Sor}
• Gene: Sptbn4 Location: Chr7:27055808-27147111 bp, - strand Genetic Position: Chr7, 15.88 cM, cytoband A3
• Alliance: Sptbn4^{Gt(ROSABetageo)62Sor} page

Mutation origin

• Mutant Cell Line: ROSA62
• Germline Transmission: Earliest citation of germline transmission: J:77228
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: ROSABetageo*
• Mutation details: A gene trap vector inserted between the exons that encode the spectrin repeat 12 of the protein. Northern blot analysis demonstrated that a fusion transcript was expressed that consisted of the gene sequences and the beta-galactosidase gene of the vector. Western blot analysis on brain lysates of homozygous mice demonstrated that no detectable full length protein was made from this allele. (J:77228)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  FHCRC-GT-ROSA62	CG465913 ; (MGI Seq Detail)	5' RACE	Chr7:27066024-27071428 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  31 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sptbn4 Mutation: 122 strains or lines available

Notes

This mutation was identified in a gene-trap screen employing a modification of the Betageo gene trap vector, originally called Gt(ROSABgeo*), encoding aspartic acid at amino acid position 182 (Komada et al. 2000).

References

• Original: J:77228 Komada M, et al., [Beta]IV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier. J Cell Biol. 2002 Jan 21;156(2):337-48
• All: 6 reference(s)