Trp53bp1^{Gt(OST94324)Lex}
Gene trapped Allele Detail

Summary

• Symbol: Trp53bp1^{Gt(OST94324)Lex}
• Name: transformation related protein 53 binding protein 1; gene trap OST94324, Lexicon Genetics
• MGI ID: MGI:2661096
• Synonyms: 53BP1^-, m53BP1^tr, Trp53bp1^{Gt(neo)531Lex}, Trp53bp1^{Gt(VICTR54)19Lex}
• Gene: Trp53bp1 Location: Chr2:121023762-121101888 bp, - strand Genetic Position: Chr2, 60.37 cM
• Alliance: Trp53bp1^{Gt(OST94324)Lex} page

Mutation origin

• Mutant Cell Line: OST94324
• Germline Transmission: Earliest citation of germline transmission: J:83164
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd
• Project Collection: Lexicon

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: The gene trap vector VICTR54 inserted into the intron between exons 13 and 14 resulting in premature stop codons downstream. RT-PCR analysis showed that exon 13 failed to properly splice next to exon 14. The expressed protein lacks over 700 residues, which include the functional nuclear localization signal, kinetochore binding domain, and 2 BRCT motifs. (J:83164)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST94324	CG523199 ; (MGI Seq Detail)	3' RACE	Chr2:121039700-121041902 bp (-)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  23 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53bp1^{Gt(OST94324)Lex}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53bp1 Mutation: 100 strains or lines available

References

• Original: J:83164 Morales JC, et al., Role for the BRCA1 C-terminal repeats (BRCT) protein 53BP1 in maintaining genomic stability. J Biol Chem. 2003 Apr 25;278(17):14971-7
• All: 9 reference(s)