Mif^tm1Mky
Targeted Allele Detail

Summary

• Symbol: Mif^tm1Mky
• Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor); targeted mutation 1, Toshifumi Mikayama
• MGI ID: MGI:2661491
• Synonyms: GIF-, MIF-
• Gene: Mif Location: Chr10:75695187-75696074 bp, - strand Genetic Position: Chr10, 38.59 cM, cytoband C1
• Alliance: Mif^tm1Mky page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75496
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of a 201 bp region spanning parts of exon 1 and intron 1 with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of brain extracts. (J:75496)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mif Mutation: 22 strains or lines available

References

• Original: J:75496 Honma N, et al., Deficiency of the macrophage migration inhibitory factor gene has no significant effect on endotoxaemia. Immunology. 2000 May;100(1):84-90
• All: 16 reference(s)