Igl^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Igl^tm1Cgn
• Name: immunoglobulin lambda chain complex; targeted mutation 1, University of Cologne
• MGI ID: MGI:2662343
• Synonyms: VJlambda1i
• Gene: Igl Location: unknown Genetic Position: Chr16, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83289
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intergenic deletion
• Mutation details: An 18 kb region between Igl-V1 and Igl-J1 was replaced with a pre-arranged VJ1 gene and a floxed ACN cassette containing a neo resistance gene and sperm-specific cre gene. The ACN cassette is excised in chimeric animals during spermatogenesis. Semiquantitative RT-PCR analysis confirmed the expression of the prearranged VJ1 insertion in splenic B cells. (J:83289)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igl Mutation: 3 strains or lines available

References

• Original: J:83289 Oberdoerffer P, et al., Expression of a targeted lambda 1 light chain gene is developmentally regulated and independent of Ig kappa rearrangements. J Exp Med. 2003 May 5;197(9):1165-72
• All: 1 reference(s)