Slit3^tm1Dor
Targeted Allele Detail

Summary

• Symbol: Slit3^tm1Dor
• Name: slit guidance ligand 3; targeted mutation 1, David M Ornitz
• MGI ID: MGI:2662346
• Synonyms: Slit3^-
• Gene: Slit3 Location: Chr11:35012283-35599334 bp, + strand Genetic Position: Chr11, 20.29 cM, cytoband A5
• Alliance: Slit3^tm1Dor page

Congenital diaphragmatic hernia (CDH) and lung pathology in Slit3^tm1Dor/Slit3^tm1Dor mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83292
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3' portion of exon 1 (194 nt) was replaced along with approximately 800 nt of intron 1 by a cassette containing Beta-gal followed by floxed neo. The deleted sequence encoded the putative signal peptide, from the start codon to the first leucine-rich repeat. Endogenous transcript was undetected in homozygous mutant mice by RNase protection. The expression pattern of Beta-gal was similar to that of the endogenous gene. (J:83292)

Disease models

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Slit3 Mutation: 81 strains or lines available

References

• Original: J:83292 Yuan W, et al., A genetic model for a central (septum transversum) congenital diaphragmatic hernia in mice lacking Slit3. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5217-22
• All: 9 reference(s)