Mylk^tm1Dmw
Targeted Allele Detail

Summary

• Symbol: Mylk^tm1Dmw
• Name: myosin, light polypeptide kinase; targeted mutation 1, D Martin Watterson
• MGI ID: MGI:2662927
• Synonyms: MLCK^-, nmmlck^-
• Gene: Mylk Location: Chr16:34565580-34822790 bp, + strand Genetic Position: Chr16, 24.49 cM
• Alliance: Mylk^tm1Dmw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83394
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Sequence specific for the endothelial cell isoform (MLCK210) was disrupted by the insertion of a neo cassette into exon 8, leaving sequence encoding the smooth muscle isoform (MLCK108) intact. Western blot analysis of homozygous mutant mice demonstrated that while MLCK210 protein is undetected, the level of MLCK108 protein is similar to that of wild-type. (J:83394)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mylk Mutation: 103 strains or lines available

References

• Original: J:83394 Wainwright MS, et al., Protein kinase involved in lung injury susceptibility: evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment. Proc Natl Acad Sci U S A. 2003 May 13;100(10):6233-8
• All: 26 reference(s)