Wasf1^{Gt(VICTR37)1Kwak}
Gene trapped Allele Detail

Summary

• Symbol: Wasf1^{Gt(VICTR37)1Kwak}
• Name: WASP family, member 1; gene trap 1, Seung P Kwak
• MGI ID: MGI:2663235
• Synonyms: Wasf1^Gt689Lex, WAVE1^-
• Gene: Wasf1 Location: Chr10:40759476-40814565 bp, + strand Genetic Position: Chr10, 22.07 cM
• Alliance: Wasf1^{Gt(VICTR37)1Kwak} page

Mutation origin

• Mutant Cell Line: OST66260
• Germline Transmission: Earliest citation of germline transmission: J:83377
• Parent Cell Line: Lex-1 (ES Cell)
• Strain of Origin: 129S5/SvEvBrd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A gene trap vector (SA-Bgeo-pA-PGK-BTK-SD) inserted in intronic sequence approximately 20 bp upstream of the endogenous start codon in exon 2. Two transcripts were identified by 5' RACE PCR, each arising from one of the two untranslated exons (1a and 1b). Protein was undetected by Western blot analysis of homozygous mutant cerebral cortex and hippocampus tissues. The pattern of beta-galactosidase activity was similar to the expression pattern of the endogenous gene. (J:83377)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  OST66260	CG513076 ; (MGI Seq Detail)	3' RACE	Chr10:40796241-40806764 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  52 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Mice Carrying this Mutation:	62 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wasf1 Mutation: 50 strains or lines available

References

• Original: J:83377 Dahl JP, et al., Characterization of the WAVE1 knock-out mouse: implications for CNS development. J Neurosci. 2003 Apr 15;23(8):3343-52
• All: 6 reference(s)