Pdgfra^{tm9(Pdgfra/Fgfr1)Sor}
Targeted Allele Detail

Summary

• Symbol: Pdgfra^{tm9(Pdgfra/Fgfr1)Sor}
• Name: platelet derived growth factor receptor, alpha polypeptide; targeted mutation 9, Philippe Soriano
• MGI ID: MGI:2663633
• Synonyms: alpha^FR
• Gene: Pdgfra Location: Chr5:75312953-75358876 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Pdgfra^{tm9(Pdgfra/Fgfr1)Sor} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83560
• Parent Cell Line: AK7 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The gene was disrupted by a knock-in vector consisting of a Pdgfra/Fgfr1 fusion protein and a PGK-neo cassette. The fusion protein encodes the Pdgfra extracellular ligand binding domain fused to the cytoplasmic domain of Fgfr1. FACS analysis confirmed cell surface expression of the chimeric protein in embryonic fibroblasts. (J:83560)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfra Mutation: 88 strains or lines available

References

• Original: J:83560 Hamilton TG, et al., Evolutionary divergence of platelet-derived growth factor alpha receptor signaling mechanisms. Mol Cell Biol. 2003 Jun;23(11):4013-25
• All: 1 reference(s)