Notch2^tm2Hhi
Targeted Allele Detail

Summary

• Symbol: Notch2^tm2Hhi
• Name: notch 2; targeted mutation 2, Hisamaru Hirai
• MGI ID: MGI:2663711
• Synonyms: Notch2^-
• Gene: Notch2 Location: Chr3:97920854-98057683 bp, + strand Genetic Position: Chr3, 42.42 cM
• Alliance: Notch2^tm2Hhi page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83476
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Flanking loxP sites and a PGK-neo cassette were introduced to the exons encoding the transmembrane domains. These exons were subsequently deleted by transient expression of cre recombinase in correctly targeted ES cells. Absence of gene expression in homozygous mutant embryos was confirmed by Western blot analysis. (J:83476)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Notch2 Mutation: 99 strains or lines available

References

• Original: J:83476 Saito T, et al., Notch2 is preferentially expressed in mature B cells and indispensable for marginal zone B lineage development. Immunity. 2003 May;18(5):675-85
• All: 2 reference(s)