Ahr^tm2Bra
Targeted Allele Detail

Summary

• Symbol: Ahr^tm2Bra
• Name: aryl-hydrocarbon receptor; targeted mutation 2, Christopher A Bradfield
• MGI ID: MGI:2663776
• Synonyms: Ahr^nls
• Gene: Ahr Location: Chr12:35547978-35584988 bp, - strand Genetic Position: Chr12, 15.78 cM
• Alliance: Ahr^tm2Bra page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83597
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Several nucleotide substitutions, resulting in amino acid substitutions R37A, H38G, and R39S, were introduced to disrupt the nuclear localization signal and the dioxin-responsive element (DRE) binding domain in exon 2. Also, a neomycin resistance gene cassette was introduced into intron 1 for positive selection. Western blot analysis confirmed expression of the mutant protein is similar that of wild-type. (J:83597)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ahr Mutation: 112 strains or lines available

References

• Original: J:83597 Bunger MK, et al., Resistance to 2,3,7,8-tetrachlorodibenzo-p-dioxin toxicity and abnormal liver development in mice carrying a mutation in the nuclear localization sequence of the aryl hydrocarbon receptor. J Biol Chem. 2003 May 16;278(20):17767-74
• All: 6 reference(s)