Dio3^tm1Stg
Targeted Allele Detail

Summary

• Symbol: Dio3^tm1Stg
• Name: deiodinase, iodothyronine type III; targeted mutation 1, Donald St Germain
• MGI ID: MGI:2664293
• Synonyms: D3KO, -/-Dio3
• Gene: Dio3 Location: Chr12:110245664-110247531 bp, + strand Genetic Position: Chr12, 60.56 cM, cytoband F1
• Alliance: Dio3^tm1Stg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80434
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: The insertion of a triple point mutation resulted in a missense mutation of the codon encoding the selenocysteine as well as of the codon immediately upstream. A floxed neo cassette was also incorporated into the untranslated region of the gene. Endogenous activity was undetected in the heads and bodies of homozygous mutant mice. The neomycin cassette was subsequently removed by crossing to a mouse carrying a cre transgene on the X chromosome. (J:80434, J:105434)

Expression

In Mice Carrying this Mutation:	120 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dio3 Mutation: 7 strains or lines available

References

• Original: J:80434 Hernandez A, et al., The gene locus encoding iodothyronine deiodinase type 3 (Dio3) is imprinted in the fetus and expresses antisense transcripts. Endocrinology. 2002 Nov;143(11):4483-6
• All: 23 reference(s)