Gnb5^tm1Ck
Targeted Allele Detail

Summary

• Symbol: Gnb5^tm1Ck
• Name: guanine nucleotide binding protein (G protein), beta 5; targeted mutation 1, Ching-Kang Chen
• MGI ID: MGI:2664296
• Synonyms: Gbeta5^-
• Gene: Gnb5 Location: Chr9:75213570-75253158 bp, + strand Genetic Position: Chr9, 42.3 cM
• Alliance: Gnb5^tm1Ck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83620
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 3 with a neomycin resistance cassette via homologous recombination. Absence of protein (both long and short forms) was confirmed by Western blot analysis of retinal extracts from homozygous mutant mice. Western blotting of mutant protein extracts also revealed loss of RGS6, RGS7, and RGS11 and a reduction of RGS9 (<5% of wild-type levels) in mutant retinas, as well as absence of RGS9 and RGS7 in mutant striatum, indicating instability of RGS proteins containing the G protein gamma-like (GGL) domain. (J:83620)

Expression

In Mice Carrying this Mutation:	2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gnb5 Mutation: 24 strains or lines available

References

• Original: J:83620 Chen CK, et al., Instability of GGL domain-containing RGS proteins in mice lacking the G protein beta-subunit Gbeta5. Proc Natl Acad Sci U S A. 2003 May 27;100(11):6604-9
• All: 20 reference(s)