Prkcq^tm1.1Bai
Targeted Allele Detail

Summary

• Symbol: Prkcq^tm1.1Bai
• Name: protein kinase C, theta; targeted mutation 1.1, Gottfried Baier
• MGI ID: MGI:2664377
• Synonyms: PKCTheta^-, PKCTheta^Delta
• Gene: Prkcq Location: Chr2:11176922-11306033 bp, + strand Genetic Position: Chr2, 8.42 cM
• Alliance: Prkcq^tm1.1Bai page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83733
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The floxed region encompassing about 10 kb of sequence, including a neo cassette and exons 3 and 4, was excised via cre mediated recombination in the germline. The deletion eliminated sequence encoding amino acids 10 through 87 and resulted in a frameshift mutation after codon 9. Western blot analysis indicated an absence of protein in thymocytes and CD3+ T cells. Sequence and splice sites corresponding to the testis-expressed isoform were left intact. (J:83733)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkcq Mutation: 51 strains or lines available

References

• Original: J:83733 Pfeifhofer C, et al., Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells. J Exp Med. 2003 Jun 2;197(11):1525-35
• All: 10 reference(s)