Cd28^tm1Pjl
Targeted Allele Detail

Summary

• Symbol: Cd28^tm1Pjl
• Name: CD28 antigen; targeted mutation 1, Philip J Lucas
• MGI ID: MGI:2664380
• Synonyms: CD28-
• Gene: Cd28 Location: Chr1:60785547-60812521 bp, + strand Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
• Alliance: Cd28^tm1Pjl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:25536
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of a 0.5 kb region containing most of exon 2 with a neomycin resistance cassette. Exon 2 encodes the Ig-like region, an alternative splice site, a putative ligand-binding domain, and represents more than 70% of the extracellular domain. Absence of gene expression was verified by flow cytometry of CD4+ T cells from homozygous mutant animals. (J:25536)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd28 Mutation: 52 strains or lines available

References

• Original: J:25536 Lucas PJ, et al., Naive CD28-deficient T cells can initiate but not sustain an in vitro antigen-specific immune response. J Immunol. 1995 Jun 1;154(11):5757-68
• All: 5 reference(s)