Hsf2^tm1Miv
Targeted Allele Detail

Summary

• Symbol: Hsf2^tm1Miv
• Name: heat shock factor 2; targeted mutation 1, Nahid F Mivechi
• MGI ID: MGI:2664486
• Synonyms: Hsf2^-
• Gene: Hsf2 Location: Chr10:57362481-57389231 bp, + strand Genetic Position: Chr10, 29.19 cM, cytoband B3-B4
• Alliance: Hsf2^tm1Miv page

Testes are smaller in Hsf2^tm1Miv/Hsf2^tm1Miv and Hsf1^tm1Miv/Hsf1^tm1Miv Hsf2^tm1Miv/Hsf2^tm1Miv mice

Show the 4 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83730
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding sequence of exon 1 was replaced with an EGFP-neo cassette which caused the termination of transcription. The neomycin resistance gene was flanked by loxP sites. Correct targeting was demonstrated by Southern blot. Transcripts were lacking in northern blots of total liver RNA from mice homozygous for the targeted allele and no Hsf2 protein expression was seen in western blots. (J:83730)

Expression

In Mice Carrying this Mutation:	48 assay results
In Structures Affected by this Mutation:	7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hsf2 Mutation: 38 strains or lines available

References

• Original: J:83730 Wang G, et al., Targeted disruption of the heat shock transcription factor (hsf)-2 gene results in increased embryonic lethality, neuronal defects, and reduced spermatogenesis. Genesis. 2003 May;36(1):48-61
• All: 3 reference(s)