Nanog^tm1Yam
Targeted Allele Detail

Summary

• Symbol: Nanog^tm1Yam
• Name: Nanog homeobox; targeted mutation 1, Shinya Yamanaka
• MGI ID: MGI:2664492
• Synonyms: Nanog^-
• Gene: Nanog Location: Chr6:122684448-122691592 bp, + strand Genetic Position: Chr6, 57.8 cM, cytoband F2
• Alliance: Nanog^tm1Yam page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83420
• Parent Cell Line: RF8 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 of the gene, which contains the homeodomain, was replaced with an IRES-Beta geo cassette. X-gal staining of preimplantation embryos detected beta-geo in the inner cell mass of blastocysts and the central portion of the morula but not in 32-cell stage embryos or earlier. (J:83420)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nanog Mutation: 47 strains or lines available

References

• Original: J:83420 Mitsui K, et al., The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003 May 30;113(5):631-42
• All: 8 reference(s)