Cd33^tm1Avrk
Targeted Allele Detail

Summary

• Symbol: Cd33^tm1Avrk
• Name: CD33 molecule; targeted mutation 1, Ajit Varki
• MGI ID: MGI:2664507
• Synonyms: Cd33^-, Cd33^tm1Ajv
• Gene: Cd33 Location: Chr7:43176823-43186679 bp, - strand Genetic Position: Chr7, 28.25 cM
• Alliance: Cd33^tm1Avrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83728
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 1 through 5 were replaced by a pPGK-neo-bpA cassette. Homologous recombination was confirmed by PCR and Southern blot. Loss of gene expression was confirmed by flow cytometric analysis of hematopoietic cells. (J:83728)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd33 Mutation: 27 strains or lines available

References

• Original: J:83728 Brinkman-Van Der Linden EC, et al., CD33/Siglec-3 Binding Specificity, Expression Pattern, and Consequences of Gene Deletion in Mice. Mol Cell Biol. 2003 Jun;23(12):4199-206
• All: 4 reference(s)