Men1^tm1Zqw
Targeted Allele Detail

Summary

• Symbol: Men1^tm1Zqw
• Name: multiple endocrine neoplasia 1; targeted mutation 1, Zhao-Qi Wang
• MGI ID: MGI:2664869
• Synonyms: Men1^T
• Gene: Men1 Location: Chr19:6385009-6390921 bp, + strand Genetic Position: Chr19, 4.45 cM, cytoband A
• Alliance: Men1^tm1Zqw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83882
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A cassette expressing the neomycin resistance gene and the thymidine kinase gene flanked by loxP sites was inserted into intron 2. An additional loxP site was placed in intron 3. Homologous recombination was confirmed by Southern blots. The insertion of a neoTK cassette into intron 2 of the Men1 locus (the T allele) disrupted the Men1 expression at RNA and protein levels, as shown by RT-PCR and Western blot, respectively. (J:83882)

Disease models

Expression

In Mice Carrying this Mutation:	3 assay results
In Structures Affected by this Mutation:	15 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Men1^tm1Zqw

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Men1 Mutation: 40 strains or lines available

References

• Original: J:83882 Bertolino P, et al., Genetic ablation of the tumor suppressor menin causes lethality at mid-gestation with defects in multiple organs. Mech Dev. 2003 May;120(5):549-60
• All: 11 reference(s)