Gjc2^tm1(EGFP)Kwi
Targeted Allele Detail

Summary

• Symbol: Gjc2^tm1(EGFP)Kwi
• Name: gap junction protein, gamma 2; targeted mutation 1, Klaus Willecke
• MGI ID: MGI:2664941
• Synonyms: Cx47^EGFP
• Gene: Gjc2 Location: Chr11:59066394-59074039 bp, - strand Genetic Position: Chr11, 37.05 cM
• Alliance: Gjc2^tm1(EGFP)Kwi page

Disturbed myelin basic protein (MBP) expression accompanied by astrogliosis and microglial activation in juvenile Gjc2^tm2.1Kwi/Gjc2^tm2.1Kwi, Gjc2^tm2.1Kwi/Gjc2⁺, and Gjc2^tm1(EGFP)Kwi/Gjc2^tm1(EGFP)Kwi mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83885
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: EGFP-1 was inserted, beginning with its ATG start codon, "in frame" after the first 7 codons of the gene followed (in antisense orientation) by HPRT driven by the mouse PGK promotor. This insertion replaced the rest of the gene and 560 bp of the 3' untranslated region. Homozygous mutant mice failed to express target gene mRNA but did express EGFP mRNA. (J:83885)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gjc2 Mutation: 22 strains or lines available

References

• Original: J:83885 Odermatt B, et al., Connexin 47 (Cx47)-deficient mice with enhanced green fluorescent protein reporter gene reveal predominant oligodendrocytic expression of Cx47 and display vacuolized myelin in the CNS. J Neurosci. 2003 Jun 1;23(11):4549-59
• All: 12 reference(s)