Ace^tm4Keb
Targeted Allele Detail

Summary

• Symbol: Ace^tm4Keb
• Name: angiotensin I converting enzyme; targeted mutation 4, Kenneth E Bernstein
• MGI ID: MGI:2665017
• Synonyms: ACE.4, KAP ACE
• Gene: Ace Location: Chr11:105858774-105880790 bp, + strand Genetic Position: Chr11, 68.84 cM
• Alliance: Ace^tm4Keb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82998
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neo cassette immediately followed by a 2.3 kb kidney androgen-regulated protein promoter was inserted downstream of the endogenous somatic promoter and upstream of exon 1. The construct was designed such that transcription of the somatic isoform would be controlled by the inserted promoter, which drives expression in proximal tubule epithelium and the uterus. Unexepectedly, expression was undetected in the absence of exogenous androgens. With exogenous androgens, renal expression was less than 2% wild type levels. (J:82998)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ace Mutation: 57 strains or lines available

References

• Original: J:82998 Cole JM, et al., New approaches to genetic manipulation of mice: tissue-specific expression of ACE. Am J Physiol Renal Physiol. 2003 Apr;284(4):F599-607
• All: 6 reference(s)