H1f4^tm2Ais
Targeted Allele Detail

Summary

• Symbol: H1f4^tm2Ais
• Name: H1.4 linker histone, cluster member; targeted mutation 2, Arthur I Skoultchi
• MGI ID: MGI:2665857
• Synonyms: H1e^-
• Gene: H1f4 Location: Chr13:23805760-23806541 bp, - strand Genetic Position: Chr13, 9.83 cM, cytoband A3.2
• Alliance: H1f4^tm2Ais page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83954
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire coding region of the gene was replaced with a PGK-neo cassette using WC1.16 cells already containing a modification of Hist1h1c. Only ES cell clones containing both of these gene modifications in cis were used to generate chimeric mice. Absence of gene products was confirmed by reverse phase HPLC. (J:83954)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H1f4 Mutation: 10 strains or lines available

References

• Original: J:83954 Fan Y, et al., H1 linker histones are essential for mouse development and affect nucleosome spacing in vivo. Mol Cell Biol. 2003 Jul;23(13):4559-72
• All: 3 reference(s)