H1f3^tm2Ais
Targeted Allele Detail

Summary

• Symbol: H1f3^tm2Ais
• Name: H1.3 linker histone, cluster member; targeted mutation 2, Arthur I Skoultchi
• MGI ID: MGI:2666743
• Synonyms: H1d^-
• Gene: H1f3 Location: Chr13:23739206-23739981 bp, + strand Genetic Position: Chr13, 9.79 cM, cytoband A2-A3
• Alliance: H1f3^tm2Ais page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83954
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding region of the gene was replaced with a PGK-puromycin resistance cassette in WEC9.6 cells in which both Hist1h1c and Hist1he had already been modified. DNA from E7.5 embryos was examined PCR to confirm correct homologous recombination. Reverse phase HPLC of extracts from E10.5 embryos proved that no gene products were present. (J:83954)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H1f3 Mutation: 8 strains or lines available

References

• Original: J:83954 Fan Y, et al., H1 linker histones are essential for mouse development and affect nucleosome spacing in vivo. Mol Cell Biol. 2003 Jul;23(13):4559-72
• All: 2 reference(s)