Aldh1a1^tm1Gdu
Targeted Allele Detail

Summary

• Symbol: Aldh1a1^tm1Gdu
• Name: aldehyde dehydrogenase family 1, subfamily A1; targeted mutation 1, Gregg Duester
• MGI ID: MGI:2667220
• Synonyms: Raldh1^-
• Gene: Aldh1a1 Location: Chr19:20470079-20620829 bp, + strand Genetic Position: Chr19, 13.91 cM
• Alliance: Aldh1a1^tm1Gdu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83957
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 11 was replaced by a PGK-neomycin gene. This results in eliminating the region containing the substrate binding pocket and the tetramerization domain in any mRNA that might be transcribed. Resulting mice were genotyped by Southern blots. Absence of protein product was established by Western blot analysis. (J:83957)

Expression

In Mice Carrying this Mutation:	37 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Aldh1a1 Mutation: 39 strains or lines available

References

• Original: J:83957 Fan X, et al., Targeted disruption of Aldh1a1 (Raldh1) provides evidence for a complex mechanism of retinoic acid synthesis in the developing retina. Mol Cell Biol. 2003 Jul;23(13):4637-48
• All: 33 reference(s)