Rasgrf1^tm1Esn
Targeted Allele Detail

Summary

• Symbol: Rasgrf1^tm1Esn
• Name: RAS protein-specific guanine nucleotide-releasing factor 1; targeted mutation1, Eugenio Santos
• MGI ID: MGI:2667231
• Synonyms: GRF1^-
• Gene: Rasgrf1 Location: Chr9:89791961-89909030 bp, + strand Genetic Position: Chr9, 47.31 cM
• Alliance: Rasgrf1^tm1Esn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83950
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 5 and 6 in the catalytic domain were replaced with a PGKneomycin cassette. This inactivates all potential splicing isoforms. Proper insertion and absence of expression were demonstrated by Sothern blot analysis and RT-PCR. Absence of protein was confirmed by immunoblotting. (J:83950)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rasgrf1 Mutation: 66 strains or lines available

References

• Original: J:83950 Font de Mora J, et al., Ras-GRF1 signaling is required for normal beta-cell development and glucose homeostasis. EMBO J. 2003 Jun 16;22(12):3039-49
• All: 8 reference(s)