Ryk^tm1Stac
Targeted Allele Detail

Summary

• Symbol: Ryk^tm1Stac
• Name: receptor-like tyrosine kinase; targeted mutation 1, Steven A Stacker
• MGI ID: MGI:2667542
• Synonyms: Ryk^-
• Gene: Ryk Location: Chr9:102712119-102785506 bp, + strand Genetic Position: Chr9, 54.72 cM
• Alliance: Ryk^tm1Stac page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84392
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 14.5 kb genomic fragment was replaced with an IRES-betageo-pA cassette that was expected to join the endogenous exon 2 splice acceptor site. The deleted region included exons 2 through 6, which encode 95% of the extracellular domain and all of the transmembrane domain. RT-PCR and Western blot analyses indicated the absence of transcript and protein in homozygous mutant mice. (J:84392)

Expression

In Mice Carrying this Mutation:	36 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Ryk Mutation: 30 strains or lines available

References

• Original: J:84392 Halford MM, et al., Ryk-deficient mice exhibit craniofacial defects associated with perturbed Eph receptor crosstalk. Nat Genet. 2000 Aug;25(4):414-8
• All: 16 reference(s)