Prkdc^tm1Fwa
Targeted Allele Detail

Summary

• Symbol: Prkdc^tm1Fwa
• Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1, Frederick W Alt
• MGI ID: MGI:2667593
• Synonyms: DNA-PKcs-, DNA-PKcs^N
• Gene: Prkdc Location: Chr16:15455730-15660099 bp, + strand Genetic Position: Chr16, 10.09 cM, cytoband B1
• Alliance: Prkdc^tm1Fwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50221
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Exon 6 was disrupted by the insertion of a neomycin selection cassette. Shortened transcipts were detected in homozygous mutant ES cells by RT-PCR analysis, however neither normal or aberrantly sized protein was detected by Western blot analysis. Assays of cell extracts indicated an ablation of endogenous protein activity. (J:50221)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkdc Mutation: 418 strains or lines available

References

• Original: J:50221 Gao Y, et al., A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination. Immunity. 1998 Sep;9(3):367-76
• All: 28 reference(s)