Mt1^tm1Rch
Targeted Allele Detail

Summary

• Symbol: Mt1^tm1Rch
• Name: metallothionein 1; targeted mutation 1, Anna E Michalska
• MGI ID: MGI:2668454
• Synonyms: MT-I -, MT-null
• Gene: Mt1 Location: Chr8:94905710-94906955 bp, + strand Genetic Position: Chr8, 46.34 cM
• Alliance: Mt1^tm1Rch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:14416
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: Both metallothionein I and II were disrupted using the same targeting construct. Exon 1 of metallothionein I was disrupted by insertion of a 20 bp synthetic oligomer just downstream from the start codon which caused a frame shift. Northern blot analysis of liver mRNA from homozygotes in which the gene was induced by intraperitoneal injection of copper indicated that transcription did take place in spite of the frame shift. However, translation of the protein product did not occur. (J:14416)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mt1 Mutation: 49 strains or lines available

References

• Original: J:14416 Michalska AE, et al., Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8088-92
• All: 26 reference(s)