Runx1^tm2Spe
Targeted Allele Detail

Summary

• Symbol: Runx1^tm2Spe
• Name: runt related transcription factor 1; targeted mutation 2, Nancy A Speck
• MGI ID: MGI:2668479
• Synonyms: Cbfa2^lz, Runx1:lacZ, Runx1^lacZ, Runx1-LacZ KI, Runx1^lz
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^tm2Spe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56184
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 10kb segment including exons 7 and 8 (but not the splice acceptor site of exon 7) was replaced with lacZ coding sequence followed by a PGK-neomycin resistance gene. A fusion protein is produced which contains the N-terminal 242 amino acids of Runx1, including its nuclear localization sequences, combined with beta-galactosidase sequences. The fusion protein lacks Runx1 function. (J:56184)

Expression

In Mice Carrying this Mutation:	316 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:56184 North T, et al., Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. Development. 1999 Jun;126(11):2563-75
• All: 30 reference(s)