Hoxb1^tm6(cre)Mrc
Targeted Allele Detail

Summary

• Symbol: Hoxb1^tm6(cre)Mrc
• Name: homeobox B1; targeted mutation 6, Mario R Capecchi
• MGI ID: MGI:2668513
• Synonyms: Hoxb1-Ires-Cre
• Gene: Hoxb1 Location: Chr11:96256578-96259082 bp, + strand Genetic Position: Chr11, 59.86 cM
• Alliance: Hoxb1^tm6(cre)Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84448
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Hoxb1^tm6(cre)Mrc expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hoxb1 (MGI:96182)

• Mutation details: An IRES-Cre cassette containing a neomycin selection gene flanked with frt sites was introduced into the 3' untranslated region downstream of the translational stop codon and upstream of the endogenous polyadenylation signal. Crossing with a flp deleter line to excise the neomycin resistance gene resulted in animals able to express Cre from the Hoxb1 locus without interfering with endogenous Hoxb1 activity. (J:84448)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hoxb1 (mouse) Summary of all recombinase alleles driven by Hoxb1. Alliance mouse Hoxb1 gene page

Expression

In Mice Carrying this Mutation:

84 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb1 Mutation: 24 strains or lines available

Notes

Cre expression under the control of the Hoxb1 promoter was used to activate reporter genes in the descendants of rhombomere 4-derived neural crest cells (r4).

• first expressed at E7 in the caudal primitive streak in mesodermal somites and caudal tail
• by 10-11 somite stage reporter activity is found in ectodermal cells of r4
• by E10.5 caudal mesoderm derivatives are found throughout the embryonic trunk and limbs extending rostrally to the caudal hindbrain
• at E11.5 cells are found in the neuroepithelium and 2nd branchial arch
• by E14.5, cells are found throughout the embryo
  • in cartilage and bone of the neck and external ear
  • cells of the tongue, salivary gland, pterygopalatine ganglia
  • cell bodies and axon tracts of the facial nerve (VII)
  • CNS localization is primarily to the 7th and 8th sensory ganglion complex and the 7th cranial nerve
    • motor neurons of the facial nerve
    • glial cells

References

• Original: J:84448 Arenkiel BR, et al., Hoxb1 neural crest preferentially form glia of the PNS. Dev Dyn. 2003 Jul;227(3):379-86
• All: 20 reference(s)