Prkg1^tm1Naw
Targeted Allele Detail

Summary

• Symbol: Prkg1^tm1Naw
• Name: protein kinase, cGMP-dependent, type I; targeted mutation 1, Hermann Nawrath
• MGI ID: MGI:2668650
• Synonyms: cGKI(-)
• Gene: Prkg1 Location: Chr19:30541889-31742433 bp, - strand Genetic Position: Chr19, 25.17 cM, cytoband C2
• Alliance: Prkg1^tm1Naw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84611
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 3' region of exon 10 was replaced with a cassette inserted by homologous recombination. Though the cassette contained sequence encoding a cre-estrogen receptor 1 fusion protein preceding neo, the fusion protein was not expressed. Endogenous protein was undetected in homozygous mutant mice. (J:84611)

Expression

In Mice Carrying this Mutation:	4 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkg1 Mutation: 60 strains or lines available

References

• Original: J:84611 Wegener JW, et al., cGMP-dependent protein kinase I mediates the negative inotropic effect of cGMP in the murine myocardium. Circ Res. 2002 Jan 11;90(1):18-20
• All: 12 reference(s)