Prkg1^tm2.1Naw
Targeted Allele Detail

Summary

• Symbol: Prkg1^tm2.1Naw
• Name: protein kinase, cGMP-dependent, type I; targeted mutation 2.1, Hermann Nawrath
• MGI ID: MGI:2668653
• Synonyms: cGKI^-, cGKI^L-
• Gene: Prkg1 Location: Chr19:30541889-31742433 bp, - strand Genetic Position: Chr19, 25.17 cM, cytoband C2
• Alliance: Prkg1^tm2.1Naw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84611
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The floxed region containing exon 10 was removed from Prkg1^tm2Naw, and the resulting allele passed through the germline. Endogenous protein was undetected in homozygous mutant mice. (J:84611)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw	involves: 129S1/Sv * 129X1/SvJ
cn2	Neurod6^tm1(cre)Kan/Neurod6^+ Prkg1^tm2Naw/Prkg1^tm2.1Naw	involves: 129S1/Sv * 129X1/SvJ
cx3	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^tm1(PRKG1*)Hfm/Tagln^+	involves: 129S1/Sv * 129X1/SvJ
cx4	Prkg1^tm2.1Naw/Prkg1^tm2.1Naw Tagln^tm2(PRKG1*)Hfm/Tagln^+	involves: 129S1/Sv * 129X1/SvJ

Phenotypes:

Affected Systems	hm1	cn2	cx3		cx4
show or hide all annotated terms Sex:
behavior/neurological		N
behavior/neurological phenotype		N
cardiovascular system	√	N
cardiovascular system phenotype		N
increased mean systemic arterial blood pressure	√
abnormal vascular smooth muscle physiology	√
digestive/alimentary system	√	N
digestive/alimentary phenotype		N
abnormal intestinal transit time	√
growth/size/body	√
enlarged spleen	√
hematopoietic system	√
enlarged spleen	√
anemia	√
abnormal erythrocyte morphology	√
decreased erythrocyte cell number	√
decreased hematocrit	√
increased mean corpuscular hemoglobin	√
increased mean corpuscular volume	√
increased red blood cell distribution width	√
reticulocytosis	√
increased erythrocyte clearance	√
homeostasis/metabolism	√
abnormal circulating hormone level	√
immune system	√
enlarged spleen	√
mortality/aging	√	N	√	√	√	√
mortality/aging		N
premature death	√		√	√	√	√
muscle	√
abnormal vascular smooth muscle physiology	√
nervous system		√
nervous system phenotype		N
reduced long-term potentiation		√
reproductive system			√	√	√	√
reduced female fertility			√		√
decreased litter size			√	√	√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkg1 Mutation: 60 strains or lines available

References

• Original: J:84611 Wegener JW, et al., cGMP-dependent protein kinase I mediates the negative inotropic effect of cGMP in the murine myocardium. Circ Res. 2002 Jan 11;90(1):18-20
• All: 21 reference(s)