Fn1^tm1Bwg
Targeted Allele Detail

Summary

• Symbol: Fn1^tm1Bwg
• Name: fibronectin 1; targeted mutation 1, Brigham and Women's Hospital
• MGI ID: MGI:2668886
• Synonyms: EIIIA^-
• Gene: Fn1 Location: Chr1:71624679-71692359 bp, - strand Genetic Position: Chr1, 36.05 cM, cytoband C1-C5
• Alliance: Fn1^tm1Bwg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:90917
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Transient cre-mediated recombination left a single loxp site in place of the exon encoding the EIIIA domain. RT-PCR indicates absence of EIIIA expression. Immunohistochemistry of mutant cells shows no staining above background. (J:90917)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fn1 Mutation: 130 strains or lines available

References

• Original: J:90917 Tan MH, et al., Deletion of the alternatively spliced fibronectin EIIIA domain in mice reduces atherosclerosis. Blood. 2004 Jul 1;104(1):11-8
• All: 5 reference(s)