Ap7q^129P3/J
QTL Variant Detail

Summary

• QTL variant: Ap7q^129P3/J
• Name: alcohol preference 7 QTL; 129P3/J
• MGI ID: MGI:2668896
• QTL: Ap7q Location: Chr7:48714845-48715077 bp Genetic Position: Chr7, cM position of peak correlated region/allele: 31.19 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: 129P3/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased intake of 10% ethanol solution compared to DBA/2J.
• Inheritance: Recessive

Notes

Candidate Genes

J:98595

Microarray analysis was used to identify genes showing alcohol-induced differential expression between inbred strains C57BL/6J and DBA/2J. These genes were then correlated to alcohol-related QTLs to identify potential candidate genes. The experiment involved identifying genes expressed differentially in alcohol-induced and non-induced animals but the following list of candidate genes are only for alcohol-induced states.

On mouse Chromosome 7 candidate genes identified for Ap7q at 25 cM are Fxyd7, Dbp (23 cM), and Kcnc1 (23.5 cM).

Mapping and Phenotype information for this QTL, its variants and associated markers

J:78215

Linkage analysis using 137 markers at an average resolution of 11 cM was performed on 169 (129P3/J x C57BL/6ByJ)F2 intercross animals to identify QTLs associated with ethanol consumption and preference. Parental strain C57BL/6ByJ exhibits greater preference and intake of ethanol compared to 129P3/J. The animals were subjected to a two-bottle test over a period of 8 days. During the first 4 days a water bottle containing 3% ethanol solution is used, and during the last 4 days a water bottle containing 10%ethanol solution is used. A previously mapped QTL, Ap3q, on mouse Chromosome 4 was detected in this study. Novel QTLs on mouse Chromosome 7 (Ap7q) and mouse Chromosome 8 (Ap8q) were identified.

Ap7q is strongly associated with 10% ethanol consumption andweakly associated with 3% ethanol consumption. Ap7q maps to 25 cM on mouse Chromosome 7 with peak linkage (LOD=4.37) at D7Mit69 and spans 11.4 cM - 25.5 cM. C57BL/6ByJ-derived alleles confer dominantly-inherited increase in 10% ethanol intake at this locus.

Ap8q is a male-specific locus strongly associated with 3% ethanol preference and consumption. Ap8q maps to 8 cM on mouse Chromosome 8 with peak linkage (LOD=3.65) at D8Mit95 and spans 0 cM - 27 cM. This locus exhibits additive inheritance with C57BL/6ByJ-derived alleles conferring increased ethanol intake.

Suggestive QTLs were detected on mouse Chromosomes 2 (50 cM, LOD=2.2 at D2Mit12), 9 (25 cM, LOD=2.62 at D9Mit25), 12 (15 cM, LOD=2.75 at D12Mit46), 13 (45 cM, LOD=1.93 at D13Mit147), 17 (30 cM, LOD=3.13 at D17Mit6), and 18 (20 cM, LOD=2.24 at D18Mit55).

References

• Original: J:78215 Bachmanov AA, et al., Voluntary ethanol consumption by mice: genome-wide analysis of quantitative trait loci and their interactions in a C57BL/6ByJ x 129P3/J F2 intercross. Genome Res. 2002 Aug;12(8):1257-68
• All: 1 reference(s)