Heal9^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Heal9^C57BL/6J
• Name: wound healing/regeneration 9; C57BL/6J
• MGI ID: MGI:2669648
• QTL: Heal9 Location: Chr18:69805276-69805416 bp Genetic Position: Chr18, cM position of peak correlated region/allele: 43.49 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• Mutation details: Heterozygosity for MRL/MpJ- and C57BL/6J-derived alleles confers increased wound healing compared to homozygosity for either parental allele. (J:82708)
• Inheritance: Other (see notes)

Notes

Heterozygosity for MRL/MpJ- and C57BL/6J-derived alleles confers increased wound healing compared to homozygosity for either parental allele.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:82708

A replicate population of 112 female and 94 male (MRL/MpJ-Fas^lpr x C57BL/6J)F2 animals was screened for 138 polymorphic markers at an average spacing of 9.5 cM +/- 6.6 cM to examine and confirm QTLs associated with wound healing. Parental strain MRL/MpJ-Fas^lpr exhibits faster wound healing after introduction of an ear hole punch compared to C57BL/6J. Interestingly, MRL/MpJ female animals exhibit faster wound healing than MRL/MpJ males, but these effects are not observed in the MRL/MpJ-Fas^lpr substrain. Several previously identified wound healing QTLs were confirmed in this study, and 3 new loci were identified.

Heal1 (Chr 8), Heal2, Heal3, and Heal7 (Ch 13), and Heal6 (Chr 7), are previously identified QTLs reaching statistical significance in this study.

03.03.2015 Curators Note: Because Heal1, Heal2, Heal3, Heal7 and Heal6 were previously mapped using an (MRL/MpJ-Fas^lpr x C57BL/6NTac)F2 intercross (J:50111, 1998) and C57BL/6NTac is a substrain of the C57BL/6J mice used here, we consider this a separate map study and have named the QTL identified here as Heal14 (Chr8), Heal17, Heal18, Heal21 (Chr13) and Heal20 (Chr7) respectively.

TABLE 1: Summary of (MRL xB6)F2

QTL Heal14 mapped between 49 cM - 68 cM on mouse Chromosome 8. Peak linkage occurred at D8Mit211 with LRS=18.8. The healing allele was inherited from the B6 parent in an additive mode of inheritance which displayed no sexual dimorphism.

QTL Heal17 mapped between 9 cM - 19 cM on mouse Chromosome 13. Peak linkage occured at D13Mit117 with LRS=15.2. The healing allele was inherited from the MRL parent in a dominant mode of inheritance. Males exhibit increased wound healing compared to females at this locus.

QTL Heal18 mapped between 48 cM - 60 cM also on mouse Chromosome 13. Peak linkage occured at D13Mit144 with LRS=14.9. The healing allele was inherited from the MRL parent in an additive mode. Male mice also exhibited increased healing at this locus compared to female mice.

QTL Heal20 mapped between 26 cM - 50 cM on mouse Chromosome 7. Peak linkage occured at D7Mit85, LRS=5.1. The healing allele was inherited from the MRL parent in an additive mode of inheritance and displayed no sexaul dimorphism.

QTL Heal21 mapped between 30 cM - 35 cM on mouse Chromosome 13. Peak linkage occured at D13Mit245, LRS=21.9. The healing allele was inherited from the MRL parent in a dominant mode of inheritance with male mice exhibiting increased healing at this locus compared to female mice.

Heal8 was previoulsy mapped to mouse Chromosome 13 using an (MRL/MpJ-Fas^lpr x C57BL/6NTac)F2 intercross (J:50111, 1998). This is a separate map study and we have named this QTL, mapping to mouse Chromosome 4, between 66 cM - 71 cM, Heal22. Peak linkage occured at D4Mit13, LRS=13.8. The MRL/MpJ derived alleles confer recessively inherited increase in wound healing with male mice exhibiting increased healing at this locus compared to female mice.

Novel QTL Heal9 mapped to 30 cM - 44 cM on mouse Chromosome 18. Peak linkage occurred at D18Mit33 (44 cM) with LRS=5.7.Interestingly, the heterozygous state confers increased wound healing compared to homozygosity for either parental allele. Females exhibit increased wound healing compared to males at Heal9.

Novel QTL Heal10mapped to 55 cM - 62 cM on mouse Chromosome 11. Peak linkage occurred at D11Mit213 (55 cM) with LRS=11. MRL/MpJ-derived alleles conferring additively inherited increase in wound healing. Males exhibit increased wound healing compared to females at Heal10.

Novel QTL Heal11 mapped to 4 cM - 20 cM on mouse Chromosome 16. Peak linkage occurred at D16Mit122 with LRS=9.1. C57BL/6J-derived alleles confer recessively inherited increase in wound healing. Male exhibit increased wound healing compared to females at Heal11.

References

• Original: J:82708 Blankenhorn EP, et al., Sexually dimorphic genes regulate healing and regeneration in MRL mice. Mamm Genome. 2003 Apr;14(4):250-60
• All: 1 reference(s)