Gpam^tm1Rcol
Targeted Allele Detail

Summary

• Symbol: Gpam^tm1Rcol
• Name: glycerol-3-phosphate acyltransferase, mitochondrial; targeted mutation 1, Rosalind Coleman
• MGI ID: MGI:2669956
• Synonyms: Gpat1^-, mtGPAT^-
• Gene: Gpam Location: Chr19:55056067-55115666 bp, - strand Genetic Position: Chr19, 50.81 cM
• Alliance: Gpam^tm1Rcol page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84541
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.5 kb genomic fragment encoding 62 amino acids including those that make up homology regions II and III was replaced with a neo cassette inserted by homologous recombination. Transcript was undetected by Northern blot analysis of homozygous mutant liver tissue. Activity assays indicated a near comlpete ablation of endogenous activity and showed that the activity of the microsomal isoform was unperturbed. (J:84541)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gpam Mutation: 53 strains or lines available

References

• Original: J:84541 Hammond LE, et al., Mitochondrial glycerol-3-phosphate acyltransferase-deficient mice have reduced weight and liver triacylglycerol content and altered glycerolipid fatty acid composition. Mol Cell Biol. 2002 Dec;22(23):8204-14
• All: 14 reference(s)