Icosl^tm1Mak
Targeted Allele Detail

Summary

• Symbol: Icosl^tm1Mak
• Name: icos ligand; targeted mutation 1, Tak W Mak
• MGI ID: MGI:2669961
• Synonyms: B7h^-, B7RP-1 KO, Icosl-, ICOSL KO
• Gene: Icosl Location: Chr10:77904921-77915359 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Icosl^tm1Mak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84668
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Most of exon 2 and all of exon 3 which together encode for the leader sequence, the first IgV domain and the ICOS-binding site were replaced with a neomycin-resistance gene. Loss of protein expression in mice homozygous for this deletion was established by flow cytometric analysis of spleen cells. (J:84668)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Icosl Mutation: 28 strains or lines available

References

• Original: J:84668 Mak TW, et al., Costimulation through the inducible costimulator ligand is essential for both T helper and B cell functions in T cell-dependent B cell responses. Nat Immunol. 2003 Aug;4(8):765-72
• All: 33 reference(s)