Skil^tm2Spw
Targeted Allele Detail

Summary

• Symbol: Skil^tm2Spw
• Name: SKI-like; targeted mutation 2, Sonia Pearson-White
• MGI ID: MGI:2670012
• Synonyms: Sno^ex1-, SnoN^-
• Gene: Skil Location: Chr3:31149259-31176741 bp, + strand Genetic Position: Chr3, 15.12 cM
• Alliance: Skil^tm2Spw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84554
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 1 was replaced with a lacZ gene together with a floxed neomycin gene construct. Full length mRNA transcript is reduced below detectable levels in homozygotes although truncated mRNA was produced. No full length protein from these mRNAs was detectable on immunoblots. (J:84554)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Skil Mutation: 39 strains or lines available

References

• Original: J:84554 Pearson-White S, et al., Defective T-cell activation is associated with augmented transforming growth factor Beta sensitivity in mice with mutations in the Sno gene. Mol Cell Biol. 2003 Aug;23(15):5446-59
• All: 2 reference(s)