Ksr1^tm1Kole
Targeted Allele Detail

Summary

• Symbol: Ksr1^tm1Kole
• Name: kinase suppressor of ras 1; targeted mutation 1, Richard Kolenick
• MGI ID: MGI:2670726
• Synonyms: ksr1^-
• Gene: Ksr1 Location: Chr11:78904266-79037233 bp, - strand Genetic Position: Chr11, 46.74 cM, cytoband B5
• Alliance: Ksr1^tm1Kole page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84644
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Sequence encoding the initiator methionine and 74 downstream amino acids encompassing 85% of the unique CA1 domain was replaced with a neomycin selection cassette. Transcript was undetected by Northern blot analysis of homozygous mutant mice. Similarly, no protein was detected in homozygotes by Western blot analysis. (J:84644)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ksr1 Mutation: 50 strains or lines available

References

• Original: J:84644 Lozano J, et al., Deficiency of kinase suppressor of Ras1 prevents oncogenic ras signaling in mice. Cancer Res. 2003 Jul 15;63(14):4232-8
• All: 4 reference(s)