Mdm2^tm1Mep
Targeted Allele Detail

Summary

• Symbol: Mdm2^tm1Mep
• Name: transformed mouse 3T3 cell double minute 2; targeted mutation 1, Mary Ellen Perry
• MGI ID: MGI:2673927
• Synonyms: mdm2^puro
• Gene: Mdm2 Location: Chr10:117524780-117546663 bp, - strand Genetic Position: Chr10, 66.32 cM, cytoband C1-C3
• Alliance: Mdm2^tm1Mep page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81065
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph)
• Mutation: Insertion
• Mutation details: Exons 7, 8, and 9 were flanked by a floxed puromycin selection cassette in intron 6 and a single loxP site in intron 9. Northern and Western blot analyses, as well as phenotypic analysis, indicated that this allele is hypomorphic. Further breeding of mice carrying this allele with cre transgenic mice led to the generation of Mdm2^tm1.1Mep and Mdm2^tm1.2Mep. (J:81065)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mdm2 Mutation: 54 strains or lines available

References

• Original: J:81065 Mendrysa SM, et al., mdm2 Is Critical for Inhibition of p53 during Lymphopoiesis and the Response to Ionizing Irradiation. Mol Cell Biol. 2003 Jan;23(2):462-72
• All: 8 reference(s)