Cacna1c^tm2Hfm
Targeted Allele Detail

Summary

• Symbol: Cacna1c^tm2Hfm
• Name: calcium channel, voltage-dependent, L type, alpha 1C subunit; targeted mutation 2, Franz Hofmann
• MGI ID: MGI:2674114
• Synonyms: L1, Line B
• Gene: Cacna1c Location: Chr6:118564201-119173851 bp, - strand Genetic Position: Chr6, 55.86 cM
• Alliance: Cacna1c^tm2Hfm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66242
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 14 and 15, which together encode the transmembrane IIS5 segment and the pore in repeat II, were flanked by loxP sites and subsequently excised in vitro cre-mediated recombination prior to germline transmission. Though RT-PCR revealed aberrant transcript in which exon 13 had spliced to a point within intron 16, no protein was detected in the hearts of homozygous mutant embryos by Western blot analysis. (J:66242)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cacna1c Mutation: 144 strains or lines available

References

• Original: J:66242 Seisenberger C, et al., Functional embryonic cardiomyocytes after disruption of the L-type alpha 1C (Cav1.2) calcium channel gene in the mouse. J Biol Chem. 2000 Dec 15;275(50):39193-9
• All: 9 reference(s)