Plpp3^tm1Stw
Targeted Allele Detail

Summary

• Symbol: Plpp3^tm1Stw
• Name: phospholipid phosphatase 3; targeted mutation 1, Colin L Stewart
• MGI ID: MGI:2674217
• Synonyms: LPP3^StwO3
• Gene: Plpp3 Location: Chr4:105014544-105089961 bp, + strand Genetic Position: Chr4, 49.18 cM
• Alliance: Plpp3^tm1Stw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84753
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The exon encoding the third outer loop of the protein (aa 214 through 268) which makes up a portion of the putative catalytic domain was removed by cre-mediated recombination. While shortened transcript was revealed by Northern blot analaysis of RNA obtained from homozygous mutant embryoid bodies, no protein was detected by Western blot analysis of primary cultured cells. Endogenous phosphatase activity was abolished in homozygous mutant embryoid bodies. (J:84753)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plpp3 Mutation: 32 strains or lines available

References

• Original: J:84753 Escalante-Alcalde D, et al., The lipid phosphatase LPP3 regulates extra-embryonic vasculogenesis and axis patterning. Development. 2003 Oct;130(19):4623-37
• All: 1 reference(s)