Dgkz^tm1Gak
Targeted Allele Detail

Summary

• Symbol: Dgkz^tm1Gak
• Name: diacylglycerol kinase zeta; targeted mutation 1, Gary A Koretzky
• MGI ID: MGI:2674915
• Synonyms: Dgkz-, Dgkzeta-, Dgkzeta KO
• Gene: Dgkz Location: Chr2:91763169-91806209 bp, - strand Genetic Position: Chr2, 50.63 cM, cytoband E1
• Alliance: Dgkz^tm1Gak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85149
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cassette containing floxed neo and IRES-GFP replaced 6.1 kb of genomic sequence encompassing exons encoding the C1 and kinase domains. Normal protein was undetected by Western blot analysis of homozygous mutant thymocytes, splencytes, and bone marrow-derived macrophages. (J:85149)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dgkz Mutation: 66 strains or lines available

References

• Original: J:85149 Zhong XP, et al., Enhanced T cell responses due to diacylglycerol kinase zeta deficiency. Nat Immunol. 2003 Sep;4(9):882-90
• All: 43 reference(s)