Pgr^tm2Omc
Targeted Allele Detail

Summary

• Symbol: Pgr^tm2Omc
• Name: progesterone receptor; targeted mutation 2, Orla M Conneely
• MGI ID: MGI:2674932
• Synonyms: PRBKO^-
• Gene: Pgr Location: Chr9:8899834-8968612 bp, + strand Genetic Position: Chr9, 2.46 cM
• Alliance: Pgr^tm2Omc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85196
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: To eliminate the translation of isoform B, the start codon of isoform B was mutated to encode leucine (ATG to CTG). A single loxP site remained in an intron after a floxed selection cassette was excised via cre mediated recombination. While isoform B was undetected by Western blot analysis of homozygous mutant uterine extracts, the presence of isoform A was confirmed. (J:85196)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pgr Mutation: 75 strains or lines available

References

• Original: J:85196 Mulac-Jericevic B, et al., Defective mammary gland morphogenesis in mice lacking the progesterone receptor B isoform. Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9744-9
• All: 7 reference(s)