Cd59a^tm1Song
Targeted Allele Detail

Summary

• Symbol: Cd59a^tm1Song
• Name: CD59a antigen; targeted mutation 1, Wen-Chao Song
• MGI ID: MGI:2675293
• Gene: Cd59a Location: Chr2:103926146-103945699 bp, + strand Genetic Position: Chr2, 54.53 cM, cytoband E2-E4
• Alliance: Cd59a^tm1Song page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76578
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2, which encodes the initiator methionine and the N-terminal signal peptide, was replaced with a neo cassette. Transcript encoding the heart and kidney isoform was undetected in homozygous mutant mice, however truncated transcript similar to the testis-specific isoform was detected. RT-PCR analsysis indicated that the mutant transcript lacked exon 2. The absence of normal protein was confirmed by FACS analysis of erthrocytes obtained from homozygous mutant mice. (J:76578)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cd59a Mutation: 19 strains or lines available

References

• Original: J:76578 Miwa T, et al., Crry, but not CD59 and DAF, is indispensable for murine erythrocyte protection in vivo from spontaneous complement attack. Blood. 2002 May 15;99(10):3707-16
• All: 15 reference(s)