Ptch1^tm1.1Bjw
Targeted Allele Detail

Summary

• Symbol: Ptch1^tm1.1Bjw
• Name: patched 1; targeted mutation 1.1, Brandon J Wainwright
• MGI ID: MGI:2675358
• Gene: Ptch1 Location: Chr13:63656142-63721274 bp, - strand Genetic Position: Chr13, 32.8 cM
• Alliance: Ptch1^tm1.1Bjw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85040
• Parent Cell Line: C1368 (ES Cell)
• Strain of Origin: 129T2/SvEms

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Floxed sequence encompassing exon 3 and and FRT-flanked neo cassette was excised from Ptch^tm1Bjw via in vivo cre-mediated recombination. Truncated transcript lacking the deleted region was detected in mutant mice by RT-PCR analysis. The mutant transcript encodes the first 117 residues followed by 24 missense residues and a nonsense mutation, putatively resulting in a protein truncated ~13 residues after the first transmembrane domain. (J:85040)

Expression

In Mice Carrying this Mutation:	31 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptch1 Mutation: 115 strains or lines available

References

• Original: J:85040 Ellis T, et al., Patched 1 conditional null allele in mice. Genesis. 2003 Jul;36(3):158-61
• All: 4 reference(s)