Dnase2b^tm1Osa
Targeted Allele Detail

Summary

• Symbol: Dnase2b^tm1Osa
• Name: deoxyribonuclease II beta; targeted mutation 1, Shigekazu Nagata
• MGI ID: MGI:2675502
• Synonyms: DLAD^-
• Gene: Dnase2b Location: Chr3:146286740-146321351 bp, - strand Genetic Position: Chr3, 72.0 cM
• Alliance: Dnase2b^tm1Osa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85215
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 3 and 4 were replaced with a neomycin selection cassette inserted by homologous recombination. Included in the deleted region were codons for 2 conserved histidine residues that are putative enzymatic active sites. Truncated transcript lacking the targeted sequence, and consequently containing a frameshift mutation, was detected in mutant mice by both Northern and RT-PCR analyses. Absence of DNAse activity under acidic conditions indicated that stable and functional protein was not produced by the mutant transcript. (J:85215)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Dnase2b Mutation: 23 strains or lines available

References

• Original: J:85215 Nishimoto S, et al., Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens. Nature. 2003 Aug 28;424(6952):1071-4
• All: 2 reference(s)