Cdk2^tm1Sgo
Targeted Allele Detail

Summary

• Symbol: Cdk2^tm1Sgo
• Name: cyclin dependent kinase 2; targeted mutation 1, Sagrario Ortega
• MGI ID: MGI:2675585
• Synonyms: Cdk2^-
• Gene: Cdk2 Location: Chr10:128533808-128540900 bp, - strand Genetic Position: Chr10, 77.13 cM, cytoband D3
• Alliance: Cdk2^tm1Sgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85230
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A neomycin resistance gene flanked by loxP sites was inserted into intron 3 while a third loxP site was inserted into intron 1, generating Cdk2^tm2Sgo. The present allele resulted from Cre recombinase-mediated excision of exons 2 and 3 (and the selection cassette), leading to a predicted reading frame shift. Gene modification was confirmed by RT-PCR and sequencing of the resulting PCR product. (J:85230)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Cdk2^tm1Sgo/Cdk2^tm1Sgo	involves: 129/Sv * CD-1
cn2	Cdk2^tm1Sgo/Cdk2^tm1Sgo Kras^tm1Bbd/Kras^+ Polr2a^tm1(cre/ERT2)Bbd/Polr2a^tm1(cre/ERT2)Bbd	involves: 129S1/Sv * 129X1/SvJ
cx3	Cdk2^tm1Sgo/Cdk2^tm1Sgo Cdk4^tm2.1Bbd/Cdk4^tm2.1Bbd	involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL
cx4	Cdk2^tm1Sgo/Cdk2^+ Cdk4^tm2.1Bbd/Cdk4^+	involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * C57BL/6 * SJL
cx5	Cdk2^tm1Sgo/Cdk2^tm1Sgo Cdk4^tm1Bbd/Cdk4^tm1Bbd Cdk6^tm1Bbd/Cdk6^tm1Bbd	involves: 129S1/Sv * 129X1/SvJ * C57BL/6
cx6	Cdk2^tm1Sgo/Cdk2^tm1Sgo Cdk6^tm1Bbd/Cdk6^tm1Bbd	involves: 129S1/Sv * 129X1/SvJ * CD-1

Phenotypes:

Affected Systems	hm1		cn2	cx3	cx4	cx5	cx6
show or hide all annotated terms Sex:
cardiovascular system				√		√
decreased myocardial fiber number						√
increased myocardial fiber size				√
thin ventricular wall				√		√
abnormal fetal cardiomyocyte proliferation				√
congestive heart failure				√
cellular	√	√		√		√	√
abnormal fetal cardiomyocyte proliferation				√
abnormal cell cycle				√
abnormal meiosis	√	√
abnormal oogenesis							√
abnormal oocyte morphology	√
decreased cell proliferation						√
decreased fibroblast proliferation				√
embryo					√	√
abnormal embryo development					√
decreased embryo size						√
endocrine/exocrine glands	√	√
absent corpus luteum	√
absent ovarian follicles	√
ovary atrophy	√
abnormal seminiferous tubule morphology		√
testicular atrophy		√
growth/size/body				√		√
decreased embryo size						√
decreased body weight				√
hematopoietic system				√		√
abnormal definitive hematopoiesis				√
abnormal common myeloid progenitor cell morphology						√
decreased erythrocyte cell number				√
liver/biliary system				√		√
liver hypoplasia				√		√
abnormal liver physiology				√
mortality/aging			√	√		√
premature death			√
lethality throughout fetal growth and development, complete penetrance						√
neonatal lethality, complete penetrance				√
embryonic lethality during organogenesis, incomplete penetrance						√
muscle				√		√
decreased myocardial fiber number						√
increased myocardial fiber size				√
abnormal fetal cardiomyocyte proliferation				√
neoplasm			√
decreased tumor incidence			√
reproductive system	√	√					√	√
abnormal female reproductive system morphology	√
absent corpus luteum	√
absent ovarian follicles	√
ovary atrophy	√
abnormal gametogenesis	√	√					√	√
abnormal meiosis	√	√
abnormal oogenesis							√
abnormal oocyte morphology	√
abnormal spermatogenesis								√
abnormal spermiogenesis		√
abnormal male reproductive system morphology		√
abnormal seminiferous tubule morphology		√
testicular atrophy		√
abnormal fertility/fecundity	√	√
infertility	√	√					√	√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdk2 Mutation: 18 strains or lines available

References

• Original: J:85230 Ortega S, et al., Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice. Nat Genet. 2003 Sep;35(1):25-31
• All: 21 reference(s)