Cdk2^tm1Sgo
Targeted Allele Detail

Summary

• Symbol: Cdk2^tm1Sgo
• Name: cyclin dependent kinase 2; targeted mutation 1, Sagrario Ortega
• MGI ID: MGI:2675585
• Synonyms: Cdk2^-
• Gene: Cdk2 Location: Chr10:128533808-128540900 bp, - strand Genetic Position: Chr10, 77.13 cM, cytoband D3
• Alliance: Cdk2^tm1Sgo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85230
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A neomycin resistance gene flanked by loxP sites was inserted into intron 3 while a third loxP site was inserted into intron 1, generating Cdk2^tm2Sgo. The present allele resulted from Cre recombinase-mediated excision of exons 2 and 3 (and the selection cassette), leading to a predicted reading frame shift. Gene modification was confirmed by RT-PCR and sequencing of the resulting PCR product. (J:85230)

Expression

In Structures Affected by this Mutation:

9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdk2 Mutation: 18 strains or lines available

References

• Original: J:85230 Ortega S, et al., Cyclin-dependent kinase 2 is essential for meiosis but not for mitotic cell division in mice. Nat Genet. 2003 Sep;35(1):25-31
• All: 21 reference(s)